Modifying Enzymes
Sigma Aldrich Fine Chemicals Biosciences Inulinase from Aspergillus niger lyophilized, powder, brown-gray, ~25 U/mg | 9025-67-6 | MFCD00217463 | 250MG
Inulinase from Aspergillus niger lyophilized, powder, brown-gray, ~25 U/mg | 9025-67-6 | MFCD00217463 | 250MG
Sigma Aldrich Fine Chemicals Biosciences Benzonase(R) endonuclease EMPROVE(R) Expert | 9025-65-4 | MFCD00131010 |
Benzonase(R) endonuclease EMPROVE(R) Expert | 9025-65-4 | MFCD00131010 |
New England Biolabs, Inc. ZraI – 200 units
A restriction endonuclease that recognizes the sequence GAC_^GTC.
Enterprise Technology Solutions 7530016006981 SKILCRAFT Avery
7530016006981 SKILCRAFT Avery Index Maker Dividers 5-Tab 11 x 8.5 White 5 Sets
XenoTech,LLC EASYCYP CYP3A5LR+ 0.5 NMOL
EasyCYP CYP3A5 Low Reductase plus b5 0.5 nmol P450 at 1 nmol/ml, 10 mg/ml protein
Aldevron Dnase I, 70ug
70ug dispense of Dnase I. DNase I nonspecifically cleaves single- or double-stranded DNA, producing di-, tri- and oligonucleotide products. DNase I is free of detectable ribonuclease activity.
Aldevron Guanylyltransferase, 1750ug
1750ug dispense of Guanylyltransferase. Guanyltransferase (GT), also known as Vaccinia Capping Enzyme (VCE), catalyzes addition of 7-methylguanylate cap structures (Cap 0) to the 5’ end of RNA in three steps:
Reactions 1 & 2: Conversion of an RNA substrate pppN(pN)n to GpppN(pN)n with increasing amounts of enzyme was visualized by gel electrophoresis (uncapped, lower band; capped, upper band). One unit (U) is the amount of enzyme activity that caps 50% of a 5’-ppp oligo in 30 minutes at 37°C.
Reaction 3: Methyl transfer from SAM to GpppN(pN)n producing SAH and cap-0 mRNA was measured using the EPIgeneousTM Methyltransferase Assay (Cisbio). One unit (U) is the amount of enzyme activity that produces 1 pmol of SAH from SAM in 30 minutes at 37°C using cap-0 substrate.
Aldevron 2'O-Methyltransferase, 3400ug
3400ug dispense of 2'O-Methyltransferase. 2'-O-Methyltransferase adds a methyl group to the 2'-OH of the first nucleotide adjacent to the m7Gppp5'N cap. 2'-O-Methyltransferase utilizes S-adenosylmethionine as the methyl group donor.
Sigma Aldrich Fine Chemicals Biosciences Isovaleryl coenzyme A lith10MG
Isovaleryl coenzyme A an intermediate of the leucine catabolism. It is synthesized from -methylbutyric acid by the action of enzyme acyl CoA synthetase. The conversion of isovaleryl-CoA to methylcrotonyl-CoA is catalyzed by the enzyme isovaleryl-CoA dehydrogenase in leucine catabolism pathway. The enzyme isovaleryl-CoA oxidase also calalyzes this conversion in fatty acid -oxidation pathway.
Sigma Aldrich Fine Chemicals Biosciences Polymyxin Acylase from Pseudomonas sp. >=0.1 units/mg solid | 111174-43-7 | MFCD00286307 | 200MG
Polymyxin Acylase from Pseudomonas sp. >=0.1 units/mg solid | 111174-43-7 | MFCD00286307 | 200MG
New England Biolabs, Inc. Micrococcal Nuclease – 320000 gel units
Micrococcal nuclease is derived from Staphylococcus aureus and is a relatively non-specific endo-exonuclease. It is purified from a recombinant E. coli strain that digests double-stranded, single-stranded, circular and linear nucleic acids. The enzyme is active in the pH range of 7.0 - 10.0, with optimal activity at pH 9.2 for both RNA and DNA substrates. Cleavage preferences have been observed at sites rich in adenylate, deoxyadenylate or thymidylate. Both DNA and RNA are degraded to 3 phosphomononucleotides and dinucleotides. Micrococcal Nuclease works well with the SimpleChIP Enzymatic Chromatin IP Kit (Agarose Beads) from Cell Signaling Technology.
